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Название: SARS- and Other Coronaviruses. Laboratory Protocols
Автор: Leen Vijgen PhD, Elien Moes, Els Keyaerts PhD, Sandra Li, Marc Van Ranst MD, PhD (auth.), Dave Cavanagh (eds.)
Издательство: Humana Press
Год: 2008
Формат: pdf
Страниц: 334
Размер: 11 Мб
Язык: английский
In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors.